Introducing plant beneficial bacteria into plant seed

Description:

Description: English: (A) Plant flowers are sprayed with a bacterial suspension. (B) The bacteria colonize flowers and the developing seeds. (C) Mature seeds are collected and endophytes stay viable during seed storage. (D) Endophytes proliferate during germination and colonize the offspring plant generation. Light microscopy images of a mature maize colonized by Paraburkholderia phytofirmans strain PsJN::gusA (E–G). The blue is due to GUS-stained bacterial cells. Strain PsJN is present inside the embryo (E,F) and in radicals (G). PsJN starts moving from embryo to germinated parts (G). F is a zoom in of the first photograph (E). Date: 23 January 2017. Source: [1] doi:10.3389/fmicb.2017.00011. Author: Birgit Mitter, Nikolaus Pfaffenbichler, Richard Flavell, Stéphane Compant, Livio Antonielli, Alexandra Petric, Teresa Berninger, Muhammad Naveed, Raheleh Sheibani-Tezerji, Geoffrey von Maltzahn and Angela Sessitsch.