Hyaloperonospora Constantinescu 2002

Summary.mw-parser-output table.commons-file-information-table,.mw-parser-output.fileinfotpl-type-information{border:1px solid #a2a9b1;background-color:#f8f9fa;padding:5px;font-size:95%;border-spacing:2px;box-sizing:border-box;margin:0;width:100%}.mw-parser-output table.commons-file-information-table>tbody>tr,.mw-parser-output.fileinfotpl-type-information>tbody>tr{vertical-align:top}.mw-parser-output table.commons-file-information-table>tbody>tr>td,.mw-parser-output table.commons-file-information-table>tbody>tr>th,.mw-parser-output.fileinfotpl-type-information>tbody>tr>td,.mw-parser-output.fileinfotpl-type-information>tbody>tr>th{padding:4px}.mw-parser-output.fileinfo-paramfield{background:#ccf;text-align:right;padding-right:0.4em;width:15%;font-weight:bold}.mw-parser-output.commons-file-information-table+table.commons-file-information-table,.mw-parser-output.commons-file-information-table+div.commons-file-information-table>table{border-top:0;padding-top:0;margin-top:-8px}@media only screen and (max-width:719px){.mw-parser-output table.commons-file-information-table,.mw-parser-output.commons-file-information-table.fileinfotpl-type-information{border-spacing:0;padding:0;word-break:break-word;width:100%!important}.mw-parser-output.commons-file-information-table>tbody,.mw-parser-output.fileinfotpl-type-information>tbody{display:block}.mw-parser-output.commons-file-information-table>tbody>tr>td,.mw-parser-output.commons-file-information-table>tbody>tr>th,.mw-parser-output.fileinfotpl-type-information>tbody>tr>td,.mw-parser-output.fileinfotpl-type-information>tbody>tr>th{padding:0.2em 0.4em;text-align:left;text-align:start}.mw-parser-output.commons-file-information-table>tbody>tr,.mw-parser-output.fileinfotpl-type-information>tbody>tr{display:flex;flex-direction:column}.mw-parser-output.commons-file-information-table+table.commons-file-information-table,.mw-parser-output.commons-file-information-table+div.commons-file-information-table>table{margin-top:-1px}.mw-parser-output.fileinfo-paramfield{box-sizing:border-box;flex:1 0 100%;width:100%}} Description: English: Epiderm of a leaf of Arabidopsis thaliana seen by cryo-scanning electron microscopy (cryo-SEM). Remark the little conidiospores of Hyaloperonospora parasitica. Français : Observation de l'épiderme d'une feuille d'Arabidopsis thaliana par microscopie électronique à balayage cryogénisée (cryo-SEM). Remarquez les minuscules conidiospores de Hyaloperonospora parasitica. Date: 2002. Source: Own work. Author: Emmanuel Boutet. Permission(Reusing this file): Own work, copyleft: Multi-license with GFDL and Creative Commons CC-BY-SA-2.5 and older versions (2.0 and 1.0).

Description: English: Conidiospores of Hyaloperonospora parasitica (downy mildew) germinating on the leaf of Arabidopsis thaliana (cf.wikipedia) observed by fluorescent microscopy. Double staining: The staining with calcofluor makes the cell walls of H. parasitica fluoresceing in blue and the staining with aniline blue staining reveal callose deposition in a blue/yellow color. From left to right: fresh conidiospore intact, opening conidiospore starting to germinate (3h after inoculation), germinated conidiospore (6h after inoculation) with a short germ tube trying to penetrate between cells of A. thaliana by doing an appressorium surrounded by callose deposition. This is the first step of the short life cycle of H. parasitica. Français : Germination de conidiospores de Hyaloperonospora parasitica (Mildiou) à la surface d'une feuille d'Arabidopsis thaliana (cf.wikipedia) observée au microscope optique à fluorescence. Double coloration : Le calcofluor rend les parois cellulaires de H. parasitica fluorescentes en bleu, tandis que le bleu d'aniline permet de visualiser la déposition de callose an bleu/jaune. De gauche à droite : jeune conidiospore intact, ouverture d'un conidiospore durant sa germination (3h après inoculation), conidiospore germé (6h après inoculation) avec un court tube germinatif qui essaye de pénétrer entre les cellules d' A. thaliana avec un appressorium entouré de dépositions de callose. C'est la première étape du cycle court de H. parasitica. Date: 2002. Source: Own work. Author: Emmanuel Boutet. Permission(Reusing this file): Own work, copyleft: Multi-license with GFDL and Creative Commons CC-BY-SA-2.5 and older versions (2.0 and 1.0).

Summary.mw-parser-output table.commons-file-information-table,.mw-parser-output.fileinfotpl-type-information{border:1px solid #a2a9b1;background-color:#f8f9fa;padding:5px;font-size:95%;border-spacing:2px;box-sizing:border-box;margin:0;width:100%}.mw-parser-output table.commons-file-information-table>tbody>tr,.mw-parser-output.fileinfotpl-type-information>tbody>tr{vertical-align:top}.mw-parser-output table.commons-file-information-table>tbody>tr>td,.mw-parser-output table.commons-file-information-table>tbody>tr>th,.mw-parser-output.fileinfotpl-type-information>tbody>tr>td,.mw-parser-output.fileinfotpl-type-information>tbody>tr>th{padding:4px}.mw-parser-output.fileinfo-paramfield{background:#ccf;text-align:right;padding-right:0.4em;width:15%;font-weight:bold}.mw-parser-output.commons-file-information-table+table.commons-file-information-table,.mw-parser-output.commons-file-information-table+div.commons-file-information-table>table{border-top:0;padding-top:0;margin-top:-8px}@media only screen and (max-width:719px){.mw-parser-output table.commons-file-information-table,.mw-parser-output.commons-file-information-table.fileinfotpl-type-information{border-spacing:0;padding:0;word-break:break-word;width:100%!important}.mw-parser-output.commons-file-information-table>tbody,.mw-parser-output.fileinfotpl-type-information>tbody{display:block}.mw-parser-output.commons-file-information-table>tbody>tr>td,.mw-parser-output.commons-file-information-table>tbody>tr>th,.mw-parser-output.fileinfotpl-type-information>tbody>tr>td,.mw-parser-output.fileinfotpl-type-information>tbody>tr>th{padding:0.2em 0.4em;text-align:left;text-align:start}.mw-parser-output.commons-file-information-table>tbody>tr,.mw-parser-output.fileinfotpl-type-information>tbody>tr{display:flex;flex-direction:column}.mw-parser-output.commons-file-information-table+table.commons-file-information-table,.mw-parser-output.commons-file-information-table+div.commons-file-information-table>table{margin-top:-1px}.mw-parser-output.fileinfo-paramfield{box-sizing:border-box;flex:1 0 100%;width:100%}} Description: X 400 English: Hyphea of Hyaloperonospora parasitica (downy mildew) growing within the leaf of a compatible (susceptible) Arabidopsis thaliana (cf.wikipedia) observed by bright-field microscopy. The staining with trypan blue makes the cytoplasm of H. parasitica dark blue. The long structure is the hyphea, whereas the little spheres are haustoria. Bigger dark spheres are Oospores, the long term storage form of H. parasitica. This is the mating step of the short life cycle of H. parasitica. Français : Hyphes de Hyaloperonospora parasitica (Mildiou) dans une feuille d'Arabidopsis thaliana (cf.wikipedia) susceptible (compatible) observée au microscope optique. La coloration au bleu de trypan donne une couleur bleue foncée au cytoplasme de H. parasitica. La longue structure est l'hyphe, tandis que les petites sphères sont les haustoria. Les sphères plus grosses et très sombres sont les Oospores, la forme de stockage à long terme de H. parasitica. C'est l'étape reproductive du cycle court de H. parasitica. Date: 2002. Source: Own work. Author: Emmanuel Boutet. Permission(Reusing this file): Creative Commons CC-BY-SA-2.5.

Description: English: Draba verna with Hyaloperonospora sp. in Szczecin, NW Poland. Date: 30 March 2021, 11:43:31. Source: Own work. Author: Krzysztof Ziarnek, Kenraiz.

Description: English: Draba verna with Hyaloperonospora sp. in Szczecin, NW Poland. Date: 30 March 2021, 11:43:51. Source: Own work. Author: Krzysztof Ziarnek, Kenraiz.