Dorylaimida

Figure 1.Longidorus carniolensis sp. n. Female: A Neck region F Habitus H Pharyngeal bulb Male: G Habitus I Pharyngeal bulb; Juveniles: B–E Habitus of first, second, third and forth juvenile stages J–M Pharyngeal bulb of first, second, third and forth juvenile stages. Scale bars: B–G 1 mm; A, H–M 100 μm.

Figure 1.Crassolabium persicum sp. n. (all images are in lateral view) A Anterior region B Lip region and amphid fovea in surface C Pharyngeal expansion D Vagina E Spicules and lateral guiding piece F Female, posterior body region G Female, anterior genital branch H Male, posterior body region I Male, entire J Female, entire.

Figure 1.Longidorus cholevae sp. n. Female: A Anterior end F Habitus I Pharyngeal bulb J Anterior genital branch Male: G Habitus H Pharyngeal bulb Juveniles: B–E Habitus of first-, second-, third- and fourth-stage juveniles. Scale-bars: A, H, I, J 50 μm; B–G 1 mm.

Figure 2.Calcaridorylaimus castaneae sp. n. Female: A Pharyngeal gland nuclei B Anterior region C Pharyngeal region D Genital system E Vulval region F Sperm cells in uterus. Scale bars: A, B, E, F – 30 μm; C, D – 50 μm.

Figure 10.Longidorus carniolensis sp. n. Juvenile: A–D Anterior region of first, second, third and forth stages H–K Pharyngeal bulb of first, second, third and forth juvenile stages M, F, G, R genital primordium of first, second, third and forth stages N, S Tail shape of first stage O, T Tail shape of second stage P, U Tail shape of third stage Q, V Tail shape of forth stage Female: E Anterior region L Pharyngeal bulb W Tail shape. Scale bar: 50 μm.

Figure 2.Crassolabium persicum sp. n. (light micrographs all in lateral view).A Female, entire B Anterior region C Pharyngo–intestinal junction D Female, genital system E Neck region F Male, entire G Male, posterior region H Vagina I Female, caudal region J Spicules K Lateral guiding piece L Oviduct–uterus junction M Lateral chord and pores. (Scale bars: A, F – 500 µm; B, H, K – 10 µm; C, G – 50 µm; D, E – 100 µm; I, J, L, M – 20 µm).

Figure 9.Longidorus cholevae sp. n. Scatter plot of the functional (˜, juveniles and adults, females in orange) and replacement (™, juveniles) odontostyle in relation to body length of the juvenile developmental stages and adults.

Figure 3.Calcaridorylaimus castaneae sp. n. Female: A Posterior region. Male: B, C Extruded spicules with supplements D Posterior region. Scale bar: A–D – 30 μm.

Figure 11.Longidorus carniolensis sp. n. Scatter plot of the functional (, dark blue) and replacement odontostyle (, light blue) in relation to the body length of the juvenile stages and adults: females (, dark blue) and males (, red), female with very short odontostyle (, green).

Figure 10.Phylogenetic relationships of Longidorus cholevae sp. n. and its closest species for the D2-D3 rDNA. Bayesian Inference strict consensus tree acquired under GTR+G model. Numbers at the nodes indicating posterior probabilities higher that 0.8 and bootstrap values more that 70% for ML and NJ are presented.

Figure 4.Calcaridorylaimus castaneae sp. n. Female: A Anterior end C Amphid F–I Tail shapes Male B Anterior end D Posterior end with extruded spicules, arrow indicating the spur E Posterior end. Scale bars: A, B, D–I – 20 μm; C – 6 μm.

Fig.1: Fotomicrografía de la región anterior de la hembra. Foto: A. Esquivel.

Figure 12.Phylogenetic tree of rDNA D2/D3 expansion region sequences of Longidorus carniolensis sp. n. from Slovenia (square mark) and sequences of closely related Longidorus species (NCBI GenBank). Sequences were analysed using Neighbour Joining Method. Bootstrap support values higher than 50% are presented.

Figure 11.Phylogenetic relationships of Longidorus cholevae sp. n. and its closest species for the partial 18S-ITS1 rDNA regions. Bayesian Inference strict consensus tree acquired under K2+G model. Numbers at the nodes indicating posterior probabilities higher that 0.8 and bootstrap values more that 70% for ML and NJ are presented.

Figure 5.Calcaridorylaimus castaneae sp. n. Female: A Entire body B Lip region D Prerectum, arrow pointing tongue-like valve E Pharyngeal bulb F Vulval region with posterior uterus G Vulval region with egg in posterior uterus H, I Vulval region J Cardia L Lateral field. Male: A Entire body C Lip region K Cardia M Supplements. Scale bars: A – 200 μm; B, C, M – 6 μm; E–L – 20 μm.

Figure 2.Longidorus carniolensis sp. n. Female: A–D Anterior ends E Vestigium in the walls of the slender part of pharynx F, G Vulval region G Anterior genital branch. Scale bars: A–I 50 μm, J 100 μm.

Figure 6.Calcaridorylaimus castaneae sp. n. Male: A Posterior end B Sperm cells in testis C–F Spicular region C Lateral piece of spicules D, E Extruded spicules, arrows pointing the spur F Spicules in the body. Scale bars: A, B – 20 μm; C–F – 18 μm.

Fig.2: Fotomicrografía de la región posterior (cola) de la hembra. Foto: A. Esquivel.

Figure 3.Longidorus carniolensis sp. n. Female: A Anterior region B–D Amphidial fovea E Vestigium F–H Vulval region I Vulval region, uterus and egg J Pharyngeal bulb, dorsal and subventral glands K, L Tail – different optical sections M Sphincter N Prerectum O–Q Variation in tail shape. Scale bars: I, N 200 μm; A–G, H–M, O–Q 50 μm.

Figure 4.Longidorus carniolensis sp. n. Male: A–E Anterior end B, D, E in sublateral view F Excretory pore and ventral pores G Partly protracted spicules H–I Tail end. Scale bar: 50 μm.

Figure 5.Longidorus carniolensis sp. n. Male: A, B Variation in tail shape. Scale bar: 50 μm.

Figure 6.Longidorus carniolensis sp. n. Male: A Anterior region B, C Head region D–G Amphidial fovea H Vestigium (white arrow), excretory pore (thick arrow) and ventral pores (slender arrows) I Ejaculatory glands (marked by arrows) J Lateral field K, L Pharyngeal bulb with glandular bodies (marked by arrows) M, N Sperm cells at different stage of development. Scale bars: A 200 μm; B–N 50 μm.

Fig.1 Fotomicrografía de la región anterior de la hembra.Foto: A. Esquivel.

Figure 7.Longidorus carniolensis sp. n. Male: A Posterior genital branch B, C, E, F Tail and copulatory apparatus – different optical sections D, G Posterior end H Rectum (marked by arrow), spicules and lateral piece I Partly protracted spicules. Scale bars: A, D, G – 200 μm; B, C, E–F, H, I – 50 μm.